Recombinant Rat sCD86 Protein(FC Tag)（PDMR100044）|-Elabscience伊莱瑞特【官网】

Recombinant Rat sCD86 Protein(FC Tag)

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货号：PDMR100044
说明书

说明书

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： Mammalian
蛋白编码： O35531

别称	Activation B7-2 antigen 3;Activation B7-2 antigen;B-lymphocyte activation antigen B7-2 2;B-lymphocyte activation antigen B7-2;B7 2;B7;B7-2;B7.2;B70;B72 antigen;BU63;CD28 antigen ligand 2 2;CD28 antigen ligand 2;Cd28l2;CD28LG2;CD86;CD86 antigen;CD86 antigen;CD86 molecule;CD86;CLS1;CTLA-4 counter-receptor B7.2 2;3;CTLA-4 counter-receptor B7.2;Early T-cell costimulatory molecule 1;ETC-1;FUN 1;FUN-1;LAB72;Ly-58;Ly58;MB7;MB7-2;Membrane glycoprotein;MGC34413;T-lymphocyte activation antigen CD86;TS/A-2
表达系统	Mammalian
序列	Val29-Ile250
蛋白编码	O35531
种属	Rat
计算分子量	49.3 kDa
表观分子量	60-80 kDa
标签	C-Fc
生物活性	Not validated for activity
纯度	> 90% as determined by reducing SDS-PAGE.
内毒素	< 1.0 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景	CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

SDS-PAGE analysis of Rat Cd86 proteins, 2 μg/lane of Recombinant Rat Cd86 proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 49.3 KD

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。