Recombinant SARS-CoV-2 NSP7 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： YP_009725303.1
| 别称 |
SARS-CoV 2 nsp7
|
| 表达系统 |
E.coli
|
| 序列 |
Ser1-Gln83
|
| 蛋白编码 |
YP_009725303.1
|
| 种属 |
SARS-CoV-2
|
| 计算分子量 |
12.3 kDa
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| 表观分子量 |
12 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 87 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, 0.01%Tween 80, pH 8.5.
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| 复溶方法 |
Not Applicable
|
| 背景 |
The ～30kb positive-stranded RNA genome of coronaviruses encodes a replication/transcription machinery that is unusually complex and composed of 16 nonstructural proteins (nsps). The four proteins nsp7 to nsp10, which are conserved among all CoVs but have no functional homologs outside of the Coronaviridae, are translated as part of the viral polyproteins pp1a and pp1ab, and the mature proteins are released by the action of the SARS-CoV protease nsp5. Hexadecamer of nsp7 and nsp8 may possess dsRNA-binding activity. SARS-CoV 2 nonstructural protein 7 (nsp7) is of interest for its potential roles in the transcription and replication of the positive-stranded viral RNA genome.
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实验操作视频
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