Recombinant SARS-CoV-2 NSP8 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： YP_009725304.1
| 别称 |
SARS-CoV 2 nsp8
|
| 表达系统 |
E.coli
|
| 序列 |
Ala1-Gln198
|
| 蛋白编码 |
YP_009725304.1
|
| 种属 |
SARS-CoV-2
|
| 计算分子量 |
25 kDa
|
| 表观分子量 |
25 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 85 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein, The nsp8 protein has been shown to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis. It forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. Nsp8 was shown to have RNA-dependent RNA polymerase (RdRp) activity that could be involved in producing primers utilized by nsp12 which is normally accepted to be the RdRp for SARS-CoV.
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实验操作视频
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