Recombinant SARS-CoV-2 Papain-Like Protease Protein
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- 表达系统： E.coli
- 蛋白编码： QHD43415.1
| 别称 |
PL-PRO;PLpro;Papain-like Protease;Replicase polyprotein 1a;pp1a
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| 表达系统 |
E.coli
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| 序列 |
Glu1564-Lys1878
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| 蛋白编码 |
QHD43415.1
|
| 种属 |
SARS-CoV-2
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| 计算分子量 |
35.8 kDa
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| 表观分子量 |
34 kDa
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| 标签 |
None
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5.
|
| 复溶方法 |
Not Applicable
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| 背景 |
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
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Rhenium(V) Complexes as Cysteine-Targeting Coordinate Covalent Warheads
IF: 7.300 -
Silver N-heterocyclic carbene complexes are potent uncompetitive inhibitors of the papain-like protease with antiviral activity against SARS-CoV-2
IF: 4.100 -
Metallodrug Profiling against SARS-CoV-2 Target Proteins Identifies Highly Potent Inhibitors of the S/ACE2 interaction and the Papain-like Protease PLpro
IF: 5.236Journal： CHEMISTRY-A EUROPEAN JOURNAL (2021) -
Gold Metallodrugs to Target Coronavirus Proteins: Inhibitory Effects on the Spike-ACE2 Interaction and on PLpro Protease Activity by Auranofin and Gold Organometallics**
IF: 4.857Journal： CHEMISTRY-A EUROPEAN JOURNAL (2020)
实验操作视频
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