Recombinant Swine CXCL10 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： NP_001008691.1
- 应用性： Cell culture
| 别称 |
10 kDa Interferon Gamma-Induced Protein;C-X-C Motif Chemokine 10;CXCL10;Gamma-IP10;INP10;IP-10;SCYB10;Small-Inducible Cytokine B10
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| 表达系统 |
E.coli
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| 序列 |
Pro 23-Ala 104
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| 蛋白编码 |
NP_001008691.1
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| 种属 |
Porcine
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| 计算分子量 |
10.1 kDa
|
| 表观分子量 |
11-17 kDa
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| 标签 |
N-His
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| 生物活性 |
Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED50 for this effect is <4 ng/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Human C-X-C Motif Chemokine Ligand 10 (CXCL10) is a non-ELR chemokine secreted by various cell types; such as monocytes; endothelial cells and fibroblasts; in response to IFN-γ. CXCL10 functions via chemokine receptor CXCR3. CXCL10 has been attributed to several roles; such as chemoattraction for activated T-lymphocytes; inhibition of angiogenesis; and antitumor activity.
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实验操作视频
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