Recombinant Swine IFN gamma protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P17803
- 应用性： Cell culture
| 别称 |
IFN-gamma;IFNG
|
| 表达系统 |
E.coli
|
| 序列 |
Gln 24-Lys 166
|
| 蛋白编码 |
P17803
|
| 种属 |
Porcine
|
| 计算分子量 |
17.7 kDa
|
| 表观分子量 |
15 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to protect PK15 cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is <40 pg/mL.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them; leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens; mitogens; or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2; FGF-basic; and EGF.
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实验操作视频
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