Recombinant Swine IL-6 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P26893
- 应用性： Cell culture
| 别称 |
IFNB;IFN-β2;BSF2;BSF-2;CDF;HGF;HPGF;HSF;IFNB2;IFN-β2;POIL2;T-cell Growth Factor (TCGF);TCGF;Interleukin-6;IL6;IL-6;Interleukin 6
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| 表达系统 |
E.coli
|
| 序列 |
Arg 31-Met 212
|
| 蛋白编码 |
P26893
|
| 种属 |
Porcine
|
| 计算分子量 |
21.9 kDa
|
| 表观分子量 |
23 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to induce proliferation in T1165.85.2.1 cells.The ED50 for this effect is <1.5 ng/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
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实验操作视频
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