Recombinant Vibrio cholerae Toxin B Protein
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- 表达系统： E.coli
- 蛋白编码： P01556
| 别称 |
Cholera enterotoxin B chain;Cholera enterotoxin gamma chain;Cholera enterotoxin subunit B;Choleragenoid;ctxB;toxB
|
| 表达系统 |
E.coli
|
| 序列 |
Thr22-Asn124
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| 蛋白编码 |
P01556
|
| 种属 |
Vibrio cholerae
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| 计算分子量 |
11.8 kDa
|
| 表观分子量 |
11 kDa
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| 标签 |
None
|
| 生物活性 |
Not validated for activity
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. Cholera enterotoxin subunit B (CTXB) pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
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实验操作视频
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