SP(Substance P) ELISA Kit
中文名称：P物质(SP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 46.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用SP抗原包被于酶标板上，实验时样品或标准品中的SP与包被的SP竞争生物素标记的抗SP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，SP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中SP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 46.88 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的SP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Nasal solitary chemosensory cells govern daily rhythm in mouse model of allergic rhinitis
IF: 11.400Journal： JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY (2024)Reactivity：MouseSample Type：serum,nasal mucosa tissue -
Zhisou powder suppresses airway inflammation in LPS and CS-induced post-infectious cough model mice via TRPA1/TRPV1 channels
IF: 5.400Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)PMID：38224794Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
SIRT-2 inhibition by AK-7 orchestrates fibrotic cascades in airways through neuroimmune interaction via TRPA1, TRPM8 and TGF-β signalling
IF: 5.300Journal： BIOCHEMICAL PHARMACOLOGY (2024)Reactivity：MouseSample Type：Bronchoalveolar lavage fluid (BALF) -
Synergistic effects of Ligilactobacillus salivarius Li01 and psyllium husk prevent mice from developing loperamide-induced constipation
IF: 5.100 -
The influence of intraoral cryotherapy on postoperative pain and substance P in symptomatic apical periodontitis: randomized clinical study
IF: 3.800Journal： Scientific Reports (2024)Reactivity：HumanSample Type：apical fluid (AF) sample -
Glycoprotein Injectable Hydrogels Promote Accelerated Bone Regeneration through Angiogenesis and Innervation
IF: 10.000 -
Stool-softening effect and action mechanism of free anthraquinones extracted from Rheum palmatum L. on water deficit-induced constipation in rats
IF: 5.400 -
Evaluation of the effectiveness and mechanism of action of the Chang-Kang-Fang formula combined with bifid triple viable capsules on diarrhea-predominant irritable bowel syndrome
IF: 5.200 -
Electroacupuncture Attenuates Neuropathic Pain in a Rat Model of Cervical Spondylotic Radiculopathy: Involvement of Spinal Cord Synaptic Plasticity
IF: 2.700Journal： Journal of Pain Research (2023)PMID：37483411Reactivity：RatSample Type：cervical nerve root -
The role of calcitonin gene-related peptide and substance P in the pathogenesis of dialysis headache
IF: 1.300
实验操作视频
