ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit
中文名称：血清素/5-羟色胺(ST/5-HT)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 15.63-1000 ng/mL
- 灵敏度： 9.38 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用ST/5-HT抗原包被于酶标板上，实验时样品或标准品中的ST/5-HT与包被的ST/5-HT竞争生物素标记的抗ST/5-HT单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，ST/5-HT浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中ST/5-HT的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 ng/mL |
| 灵敏度 | 9.38 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的ST/5-HT，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Maternal LPS Exposure Enhances the 5-HT Level in the Prefrontal Cortex of Autism-like Young Offspring
IF: 3.300 -
Electroacupuncture promotes synaptic plasticity in rats with chronic inflammatory pain–related depression by upregulating BDNF/TrkB/CREB signaling pathway
IF: 3.100 -
Aerobic exercise improves ejaculatory behaviors and complements dapoxetine treatment by upregulating the BDNF-5-HT duo: a pilot study in rats
IF: 2.900Journal： ASIAN JOURNAL OF ANDROLOGY (2023)PMID：36891937Reactivity：RatSample Type：raphe nucleus samples -
The effects of acupuncture on depression by regulating BDNF-related balance via lateral habenular nucleus BDNF/TrkB/CREB signaling pathway in rats
IF: 2.700 -
PI3K-AKT-mTOR signaling pathway regulates autophagy of hippocampal neurons in diabetic rats with chronic unpredictable mild stress
IF: 2.700 -
The Role of Dihydroresveratrol in Enhancing the Synergistic Effect of Ligilactobacillus salivarius Li01 and Resveratrol in Ameliorating Colitis in Mice.
IF: 11.036 -
Decreased peripheral mtDNA in methamphetamine use disorder
IF: 10.372 -
Lactobacillus rhamnosus GG colonization in early life regulates gut-brain axis and relieves anxiety-like behavior in adulthood
IF: 10.334 -
Chaihujialonggumulitang shows psycho-cardiology therapeutic effect on acute myocardial infarction with comorbid anxiety by the activation of Nrf2/HO-1 pathway and suppression of oxidative stress and apoptosis
IF: 7.419Journal： BIOMEDICINE & PHARMACOTHERAPY (2022)PMID：36076489Reactivity：RatSample Type：hippocampus tissue -
Attenuation of hyperglycemia-associated dyslipidemic, oxidative, cognitive, and inflammatory crises via modulation of neuronal ChEs/NF-κB/COX-2/NOx, and?hepatorenal functional deficits by the Tridax procumbens extract
IF: 7.419
实验操作视频
