Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

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中文名称：总谷胱甘肽（T-GSH）/氧化型谷胱甘肽（GSSG）比色法测试盒

货号：E-BC-K097-S
文献（55）
说明书

说明书

Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Price: ¥1100.00 ¥600.00

Size:

100 Assays (96 samples) 50 Assays (48 samples)

样本类型： 血清（浆）;动物组织;红细胞;细胞
检测仪器及波长： 紫外-可见光分光光度计（412 nm）
研究领域： 氧化应激

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	通过谷胱甘肽还原酶把GSSG还原成GSH，而GSH可以和生色底物DTNB反应产生GSSG和黄色的TNB，总谷胱甘肽（GSSG+GSH）的量决定了黄色的TNB形成量。从而通过测定A412（波长在412 nm处时的吸光度值）就可以计算出总谷胱甘肽的量。再用适当试剂先清除样品中的GSH然后利用上述反应原理就可以测定出GSSG的含量。
指标简称	T-GSH
灵敏度	0.12 μmol/L T-GSH
检测范围	0.12-30 μmol/L T-GSH
精密度	批间差：4.7% \| 批内差：0.9%
样本量	100 μL
检测时间	3 h
检测仪器及波长	紫外-可见光分光光度计（412 nm）
保存条件	该产品可在 2-8°C/-20°C避光条件下保存6个月，具体试剂组分的保存条件请参考说明书。
有效期	6个月

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