VD3(Vitamin D3) ELISA Kit
中文名称:维生素D3(VD3)酶联免疫吸附测定试剂盒

Price:
- 反应性: Universal
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用VD3抗原包被于酶标板上,实验时样品或标准品中的VD3与包被的VD3竞争生物素标记的抗VD3单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,VD3浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中VD3的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Universal |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的VD3,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats
IF: 3.200Journal: Frontiers in Physiology (2024)Reactivity:RatSample Type:serum -
Clinical significance of vit D and AMH and its correlation with polycystic ovarian parameters in obese and non-obese Egyptian women
IF: 2.800 -
Lactobacillus rhamnosus GG Promotes Intestinal Vitamin D Absorption by Upregulating Vitamin D Transporters in Senile Osteoporosis
IF: 4.000 -
Alterations in the composition of the gut microbiota affect absorption of cholecalciferol in severe osteoporosis
IF: 2.976Journal: JOURNAL OF BONE AND MINERAL METABOLISM (2022)PMID:35103840Reactivity:HumanSample Type:plasma -
Correlation of Vitamin D3, PAI-1, and HCG Hormone in Pre- and Post-Menopausal in Babylon Province
Journal: Reports of Biochemistry and Molecular Biology (2022)PMID:35765537Reactivity:HumanSample Type:serum -
Is there any association between vitamin D levels and polycystic ovary syndrome (PCOS) phenotypes?
IF: 1.571Journal: Archives of Endocrinology Metabolism (2019)PMID:31576965Reactivity:HumanSample Type:serum