VD(Vitamin D) ELISA Kit
中文名称：维生素D(VD)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 6.25-400 ng/mL
- 灵敏度： 3.75 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用VD抗原包被于酶标板上，实验时样品或标准品中的VD与包被的VD竞争生物素标记的抗VD单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，VD浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中VD的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 6.25-400 ng/mL |
| 灵敏度 | 3.75 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的VD，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Phytochemical Composition Antioxidant and Anti-Inflammatory Activity of Artemisia dracunculus and Artemisia abrotanum
IF: 6.000 -
Dynamic Changes in Adiponectin and Resistin Drive Remission of Cardiometabolic Risk Biomarkers in Individuals with Obesity Following Bariatric Surgery
IF: 4.600 -
Impact of Body Mass Index Elevation, Vitamin D Receptor Polymorphisms and Antipsychotics on The Risk of Vitamin D Deficiency in Schizophrenia Patients
IF: 3.700Journal： JOURNAL OF PSYCHIATRIC RESEARCH (2024)Reactivity：HumanSample Type：Serum -
Assessing seasonal variations of biomarkers in inflammatory bowel disease
IF: 2.300Journal： EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY (2024)Reactivity：HumanSample Type：Serum -
Unveiling Role of Serum Total Bile Acid in Diabetic Osteopathy, the Unexplored Complication of Diabetes
IF: 1.500Journal： Indian Journal of Clinical Biochemistry (2024)Reactivity：HumanSample Type：serum -
Relationship of Serum 25-Hydroxyvitamin D Levels with Sarcopenia and Body Composition in Community-Dwelling Older Adults: A?Paired Case-Control Study
IF: 7.600Journal： Journal of the American Medical Directors Association (2023)PMID：37423260Reactivity：HumanSample Type：serum -
The Effects of 12 Weeks Colostrum Milk Supplementation on the Expression Levels of Pro-Inflammatory Mediators and Metabolic Changes among Older Adults: Findings from the Biomarkers and Untargeted Metabolomic Analysis
IF: 5.900 -
The Effects of Vitamin D on Movement and Cognitive Function in Senile Mice After Sevoflurane Anaesthesia
IF: 1.800Journal： EXPERIMENTAL AGING RESEARCH (2023)Reactivity：MouseSample Type：blood -
Vitamin D protects silica particles induced lung injury by promoting macrophage polarization in a KLF4-STAT6 manner
IF: 6.117Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022)PMID：36049670Reactivity：MouseSample Type：serum -
Upregulation of autophagy in M2 macrophage by vitamin D alleviates crystalline silica-induced pulmonary inflammatory damage
IF: 6.291Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021)PMID：34478973Reactivity：MouseSample Type：lung
实验操作视频
