Acetylcholinesterase (AchE) Activity Assay Kit
中文名称:乙酰胆碱酯酶(AchE)比色法测试盒

Price:
Size:
96 T (96 samples)
48 T (48 samples)
- 样本类型: 血清(浆);动物组织;细胞
- 检测仪器及波长: 酶标仪(最佳检测波长为412 nm)
- 研究领域: 氨基酸与蛋白
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
乙酰胆碱酯酶(AchE)催化乙酰胆碱(Ach)水解生成胆碱,胆碱与二硫对硝基苯甲酸(DTNB)作用生成5-巯基-硝基苯甲酸(TNB);TNB在412 nm处有特征吸收峰,通过测定412 nm处吸光度增加速率,计算AchE的活性。
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指标简称 | AchE |
灵敏度 | 1.225 U/mL |
检测范围 | 1.225-490 U/mL |
精密度 | 批间差:9.3% | 批内差:4.7% |
检测时间 | 20 min |
检测仪器及波长 | 酶标仪(最佳检测波长为412 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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