AF/LE Purified Anti-Human CD8a Antibody[OKT-8]
![AF/LE Purified Anti-Human CD8a Antibody[OKT-8]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μg
25 μg
- 应用性: FCM
- 亚型: Mouse IgG2a, κ
- 宿主: Mouse
Alternate Names | CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2 |
Clone No | |
Isotype | Mouse IgG2a, κ |
Leadtime |
咨询
|
Verified Samples | Verified Samples in FCM: Human peripheral blood lymphocytes |
Dilution |
FCM 2 μg/mL(1×105-5×105 cells)
|
Background |
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
|
Immunogen | Recombinant Human CD8 protein |
Abbre | CD8 |
Swissprot | |
Host | Mouse |
Reactivity | Human |
Clonality | Monoclonal |
Purification Method | >98%, Protein A/G purified |
Applications | |
Research Areas | Immunology |
Concentration | ≥ 1 mg/mL |
Conjugation | None (AF/LE) |
Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
Expiration Date | 12个月 |
Shipping |
Ice bag |
Human peripheral blood lymphocytes were stained with 0.2 μg AF/LE Purified Anti-Human CD8a Antibody[OKT-8] (Right) and 0.2 μg Mouse IgG2a, κ Isotype Control (Left), followed by Alexa Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody.