Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
中文名称：谷丙转氨酶（ALT/GPT）比色法测试盒
Price:
Size:
100 Assays (44 samples)
50 Assays (19 samples)
- 样本类型： 血清（浆）;动物组织;细胞
- 检测仪器及波长： 紫外-可见光分光光度计（505 nm）
- 研究领域： 肝肾功能
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
在37°C、pH7.4条件下，谷丙转氨酶（ALT）催化丙氨酸与α-酮戊二酸之间的氨基转换反应，生成丙酮酸和谷氨酸，反应时间过后，加入苯肼，与丙酮酸生成苯腙，苯腙在碱性条件下呈红棕色。
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| 指标简称 | ALT |
| 灵敏度 | 1.26 IU/L |
| 检测范围 | 1.26-72.3 IU/L |
| 精密度 | 批间差：8.2% | 批内差：4.3% |
| 样本量 | 0.2 mL |
| 检测时间 | 1 h 40 min |
| 检测仪器及波长 | 紫外-可见光分光光度计（505 nm） |
| 保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
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Q1:组织样本冻存比较久了，能否检测AST/ALT？
建议尽量使用新鲜的样本，如果没有新鲜样本，建议样本-80℃冻存不超过一个月，否则AST/ALT酶活降低，对测值有较大的影响。
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