Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)

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中文名称：碱性磷酸酶（ALP）比色法测试盒（对硝基苯磷酸二钠法）

货号：E-BC-K009-M
文献（70）
说明书

说明书

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)

Price: ¥320.00 ¥260.00

Size:

96 T (79 samples) 48 T (31 samples)

样本类型： 血清（浆）;组织;细胞
检测仪器及波长： 酶标仪（400-415 nm，最佳检测波长为405 nm）
研究领域： 肝肾功能

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	在碱性条件下，碱性磷酸酶催化对硝基苯磷酸二钠水解，生成对硝基苯酚和磷酸。对硝基苯酚在强碱性条件下呈亮黄色，在405 nm处有强烈的吸收峰。因此可以测定405 nm 处的吸收值来计算产物的生成量，从而计算出酶活。
指标简称	ALP
灵敏度	0.27 U/L
检测范围	0.27-50.8 U/L
精密度	批间差：4.6% \| 批内差：1.2%
样本量	50 μL
检测时间	30 min
检测仪器及波长	酶标仪（400-415 nm，最佳检测波长为405 nm）
保存条件	该产品可在-20°C避光条件下保存12个月。
有效期	12个月

Pterostilbene-isothiocyanate impedes RANK/TRAF6 interaction to inhibit osteoclastogenesis, promoting osteogenesis in vitro and alleviating glucocorticoid induced osteoporosis in rats

IF: 6.100

Journal： BIOCHEMICAL PHARMACOLOGY (2022)

DOI：10.1016/j.bcp.2022.115284

PMID：36209841

Reactivity：Mouse,Human,Rat

Sample Type：serum
Uncovering the Pharmacological Mechanisms of Gexia-Zhuyu Formula (GXZY) in Treating Liver Cirrhosis by an Integrative Pharmacology Strategy

IF: 5.988

Journal： Frontiers in Pharmacology (2022)

DOI：10.3389/fphar.2022.793888

PMID：35330838

Reactivity：Rat

Sample Type：serum
Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

IF: 4.996

Journal： Scientific Reports (2022)

DOI：10.1038/s41598-022-21816-x

PMID：36396948

Reactivity：Human

Sample Type：serum
Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis

IF: 3.263

Journal： BMC Endocrine Disorders (2022)

DOI：10.1186/s12902-022-01103-6

PMID：35869471

Reactivity：Mouse
San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

IF: 2.650

Journal： Evidence-based Complementary and Alternative Medicine (2022)

DOI：10.1155/2022/7832540

PMID：35845569

Reactivity：Rat

Sample Type：hepatic tissue , serum
Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion

IF: 2.623

Journal： Dose-Response (2022)

DOI：10.1177/15593258221114184

PMID：35832769

Reactivity：Rat

Sample Type：serum
miR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP

IF: 2.562

Journal： BMC MUSCULOSKELETAL DISORDERS (2022)

DOI：10.1186/s12891-022-05731-w

PMID：35978328

Reactivity：Human
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

IF: 1.628

Journal： Journal of Oleo Science (2022)

DOI：10.5650/jos.ess22033

PMID：35965085

Reactivity：Rat

Sample Type：liver
Indole-3-Carboxaldehyde Restores Gut Mucosal Integrity and Protects from Liver Fibrosis in Murine Sclerosing Cholangitis

IF: 6.600

Journal： Cells (2021)

DOI：10.3390/cells10071622

PMID：34209524

Reactivity：Mouse

Sample Type：serum
Phillygenin Attenuates Carbon Tetrachloride-Induced Liver Fibrosis via Modulating Inflammation and Gut Microbiota

IF: 5.811

Journal： Frontiers in Pharmacology (2021)

DOI：10.3389/fphar.2021.756924

PMID：34621179

Reactivity：Mouse

Sample Type：serum

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