Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)

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中文名称：碱性磷酸酶（ALP）比色法测试盒（对硝基苯磷酸二钠法）

货号：E-BC-K009-M
文献（70）
说明书

说明书

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)

Price: ¥320.00 ¥260.00

Size:

96 T (79 samples) 48 T (31 samples)

样本类型： 血清（浆）;组织;细胞
检测仪器及波长： 酶标仪（400-415 nm，最佳检测波长为405 nm）
研究领域： 肝肾功能

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	在碱性条件下，碱性磷酸酶催化对硝基苯磷酸二钠水解，生成对硝基苯酚和磷酸。对硝基苯酚在强碱性条件下呈亮黄色，在405 nm处有强烈的吸收峰。因此可以测定405 nm 处的吸收值来计算产物的生成量，从而计算出酶活。
指标简称	ALP
灵敏度	0.27 U/L
检测范围	0.27-50.8 U/L
精密度	批间差：4.6% \| 批内差：1.2%
样本量	50 μL
检测时间	30 min
检测仪器及波长	酶标仪（400-415 nm，最佳检测波长为405 nm）
保存条件	该产品可在-20°C避光条件下保存12个月。
有效期	12个月

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IF: 5.555

Journal： Frontiers in Endocrinology (2021)

DOI：10.3389/fendo.2021.759843

PMID：34777254

Reactivity：Rat

Sample Type：serum
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IF: 4.398

Journal： BONE (2021)

DOI：10.1016/j.bone.2021.116211

PMID：34560308

Reactivity：Mouse

Sample Type：serum
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IF: 4.003

Journal： RARE METALS (2021)

DOI：10.1007/s12598-021-01814-0

Reactivity：Human
Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

IF: 3.046

Journal： NEUROSCIENCE LETTERS (2021)

DOI：10.1016/j.neulet.2021.136385

PMID：34871743

Reactivity：Rat

Sample Type：serum
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IF: 2.102

Journal： DENTAL MATERIALS JOURNAL (2021)

DOI：10.4012/dmj.2020-307

PMID：33814533

Reactivity：Human

Sample Type：Cell lysate
Supplementation of non-fermented and fermented goji berry (Lycium barbarum) improves hepatic function and corresponding lipid metabolism via their anti-inflammatory and antioxidant properties in high fat-fed rats

IF: 1.813

Journal： Applied Biological Chemistry (2021)

DOI：10.1186/s13765-021-00642-1

Reactivity：Rat

Sample Type：serum
The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis

IF: 9.038

Journal： JOURNAL OF HAZARDOUS MATERIALS (2020)

DOI：10.1016/j.jhazmat.2020.124876

PMID：33360192

Reactivity：Tadpole
Noninvasive Visualization of Obesity-Boosted Inflammation in Orthotopic Pancreatic Ductal Adenocarcinoma Using an Octapod Iron Oxide Nanoparticle

Journal： ACS Applied Bio Materials (2020)

DOI：10.1021/acsabm.0c00841

PMID：35021772

Reactivity：Mouse

Sample Type：Serum
A prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells

IF: 12.804

Journal： Science Advances (2019)

DOI：10.1126/sciadv.aax0064

PMID：31457098

Reactivity：Mouse

Sample Type：serum
Isopsoralen regulates PPAR?γ/WNT to inhibit oxidative stress in osteoporosis

IF: 1.692

Journal： Molecular Medicine Reports (2017)

DOI：10.3892/mmr.2017.7954

PMID：29115612

Reactivity：Rat

Sample Type：Serum

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