Alkaline Phosphatase (ALP) Activity Assay Kit
中文名称:碱性磷酸酶(ALP)比色法测试盒

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);组织;细胞
- 检测仪器及波长: 酶标仪(500-530 nm,最佳检测波长为520 nm)
- 研究领域: 肝肾功能
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
在pH=10的反应液中,碱性磷酸酶催化磷酸苯二钠水解,生成游离酚和磷酸。酚在碱性溶液中与4-氨基安替吡啉结合,并经铁氰化钾氧化生成红色的醌的衍生物,根据红色的深浅计算酶活的高低。
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指标简称 | ALP |
灵敏度 | 0.13 King unit/100 mL |
检测范围 | 0.13-50 King unit/100 mL |
精密度 | 批间差:8.5% | 批内差:5.1% |
样本量 | 5 μL |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(500-530 nm,最佳检测波长为520 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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