Alkaline Phosphatase (ALP) Activity Assay Kit
中文名称：碱性磷酸酶（ALP）比色法测试盒
Price:
Size:
100 Assays (96 samples)
50 Assays (48 samples)
- 样本类型： 血清（浆）;组织;细胞
- 检测仪器及波长： 紫外-可见光分光光度计（520 nm）
- 研究领域： 肝肾功能
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
在pH=10的反应液中，碱性磷酸酶催化磷酸苯二钠水解，生成游离酚和磷酸。酚在碱性溶液中与4-氨基安替吡啉结合，并经铁氰化钾氧化生成红色的醌的衍生物，根据红色的深浅计算酶活的高低。
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| 指标简称 | ALP |
| 灵敏度 | 0.2 King unit/100 mL |
| 检测范围 | 0.2-55.6 King unit/1 |
| 精密度 | 批间差：5.6% | 批内差：2.1% |
| 样本量 | 50 μL |
| 检测时间 | 1 h |
| 检测仪器及波长 | 紫外-可见光分光光度计（520 nm） |
| 保存条件 | 该产品可在 2-8°C避光条件下保存3个月。 |
| 有效期 | 3个月 |
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| E-BC-K094-S | Acid Phosphatase (ACP) Activity Assay Kit | 100 Assays (96 samples) , 50 Assays (48 samples) |
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