alpha Tubulin Monoclonal Antibody-Elabscience伊莱瑞特【官网】

alpha Tubulin Monoclonal Antibody

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货号：AN005320L
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说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Mouse
反应性： Human;Mouse;Rat
应用性： WB;IF

Verified Samples	Verified Samples in WB: HeLa, MCF-7, HT-29, Hep G2, Mouse brain, Mouse kidney, Rat brain Verified Samples in IF: U-2 OS
Dilution	WB 1:2000-1:4000, IF 1:200-1:400
Clonality	Monoclonal
Isotype	IgG
Immunogen	Recombinant human TUBA1B protein by E.coli
Abbre	alpha Tubulin
Synonyms	Alpha tubulin 3, Alpha-tubulin 3, B alpha 1, FLJ25113, LIS3, TBA1A, TUBA1A, TUBA3
Swissprot	P68363
Calculated MW	55 kDa
Observed MW	55 kDa
Cellular Localization	Cytoplasm, cytoskeleton
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Protein A/G Purification
Research Areas	Signal Transduction, Neuroscience, Tags and Cell Markers, Isotype/Loading Controls, Biochemicals, Cancer, Cardiovascular, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Immunology, Metabolism, Microbiology, Stem Cells
Clone No.	2H2
Conjugation	Unconjugated
Background	Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers .Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms.Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Western blot with Anti alpha Tubulin Monoclonal Antibody at dilution of 1:3000. Lane 1: HeLa cell lysate, Lane 2: MCF-7 cell lysate, Lane 3: HT-29 cell lysate, Lane 4: Hep G2 cell lysate, Lane 5: Mouse brain tissue lysate, Lane 6: Mouse kidney tissue lysate, Lane 7: Rat brain tissue lysate.

Immunofluorescence analysis of U-2 OS cells using alpha Tubulin Monoclonal Antibody at dilution of 1:200.