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APC Anti-Human IL-4 Antibody[MP4-25D2]

APC Anti-Human IL-4 Antibody[MP4-25D2]
  • APC Anti-Human IL-4 Antibody[MP4-25D2]

Price: ¥2640.00 ¥912.00 ¥3960.00

Size:
100 Tests 20 Tests 100 Tests × 2
  • 应用性: ICFCM
  • 亚型: Rat IgG1, κ
  • 宿主: Rat
Alternate Names
B-cell IgG differentiation factor, B-cell growth factor 1, BSF-1, IGG1 induction factor, IL-4, Interleukin-4
Cellular Localization
Secreted
Clone No
Isotype
Rat IgG1, κ
Leadtime
咨询
Background
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
Abbre
IL-4
Swissprot
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype Control
Applications
Form
Liquid
Concentration
5 μL/Test
Conjugation
APC
Conjugation Information
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
Spectrum
Storage Buffer
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration Date
12个月
Shipping
冰袋
Gene ID
E-CK-A105 10×ACK Lysis Buffer 500 mL , 200 mL , 100 mL
E-CK-A107 Cell Staining Buffer 500 mL , 200 mL , 100 mL
E-CK-A109 Intracellular Fixation/Permeabilization Buffer Kit 500 Assays , 100 Assays , 50 Assays
E-CK-A091 Cell Stimulation and Protein Transport Inhibitor Kit 500 Assays , 200 Assays , 100 Assays , 50 Assays

Q11:IHC实验,想知道抗原修复条件是什么?是用EDTA还是枸橼酸钾?

修复条件一般为高温高压或者微波加热修复。
例如:抗原修复(高压法) :压力锅中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液(pH 6.0) (配制:将抗原修复液溶于相应体积 ddH2O中,混匀),加热至沸腾,切片置耐热塑料切片架上,放入锅中,盖好锅盖,扣上压力阀,继续加热,设置保压 4 min,时间到后打开放气阀放气,压力归零后开锅盖将内锅取出放置室温冷却,待溶液冷却至室温后取出切片(约30 min)。
抗原修复(微波法) :烧杯中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液(pH 6.0) (配制:将抗原修复液溶于相应体积 ddH2O中,混匀),加热至沸腾,切片置耐热塑料切片架上,放入烧杯中,中小火加热30min后取出,放置室温冷却,待溶液冷却至室温后取出切片(约30 min)。
IHC修复液的选择建议参考抗体使用说明书,或者参考以下方案:人体样本,优先推荐使用pH9.0的EDTA修复液(临床样本);大/小鼠样本,优先推荐使用pH6.0的枸橼酸修复液(科研样本)。

Q12:IHC在数据表上是什么意思?是冷冻切片吗?与IHC-F区别?

IHC是免疫组化实验,使用的切片一般包含有IHC-P(石蜡切片)和IHC-F(冰冻切片)两种。

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