APC Anti-Mouse CD206/MMR Antibody[C068C2]（E-AB-F1135UE）|-Elabscience伊莱瑞特【官网】

APC Anti-Mouse CD206/MMR Antibody[C068C2]

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货号：E-AB-F1135UE
文献（31）
说明书

说明书

APC Anti-Mouse CD206/MMR Antibody[C068C2]

Price: ¥3600.00 ¥1296.00

Size:

100 μg 25 μg

应用性： FCM;ICFCM
亚型： Rat IgG2a, κ
宿主： Rat

Alternate Names	MMR, MR, MRC1, macrophage mannose receptor, mannose receptor
Cellular Localization	Membrane;Endosome
Clone No	C068C2
Isotype	Rat IgG2a, κ
Leadtime	咨询
Background	CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.
Abbre	CD206
Swissprot	Q61830
Host	Rat
Reactivity	Mouse
Clonality	Monoclonal
Isotype Control	APC Rat IgG2a, κ Isotype Control[2A3]
Applications	FCM;ICFCM
Form	Liquid
Concentration	0.2 mg/mL
Conjugation	APC
Conjugation Information	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
Spectrum
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage	This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration Date	12个月
Shipping	冰袋
Gene ID	17533

C57BL/6 murine abdominal macrophages elicited by starch broth are stained with FITC Anti-Mouse F4/80 Antibody and APC Anti-Mouse CD206 Antibody (Left). Abdominal macrophages are stained with FITC Anti-Mouse F4/80 Antibody and APC Rat IgG2a, κ Isotype Control (Right).

Metabolism/Immunity Dual-Regulation Thermogels Potentiating Immunotherapy of Glioblastoma Through Lactate-Excretion Inhibition and PD-1/PD-L1 Blockade

IF: 14.3

Journal： Advanced Science (2024)

DOI：10.1002/advs.202310163

Reactivity：Mouse

Sample Type：GL261 cell-Xenograft
Pregnane X Receptor Activation in Liver Macrophages Protects against Endotoxin-Induced Liver Injury

IF: 14.3

Journal： Advanced Science (2024)

DOI：10.1002/advs.202308771

Reactivity：Mouse
Dual-Drug Nanomedicine Assembly with Synergistic Anti-Aneurysmal Effects via Inflammation Suppression and Extracellular Matrix Stabilization

IF: 13.0

Journal： Small (2024)

DOI：10.1002/smll.202402141

Reactivity：Mouse
Legumain-Guided Ferulate-Peptide Self-Assembly Enhances Macrophage-Endotheliocyte Partnership to Promote Therapeutic Angiogenesis After Myocardial Infarction

IF: 10.0

Journal： Advanced Healthcare Materials (2024)

DOI：10.1002/adhm.202402056

Reactivity：Mouse
Osteosarcoma-targeting PtIV prodrug amphiphile for enhanced chemo-immunotherapy via Ca2+ trapping

IF: 9.4

Journal： Acta Biomaterialia (2024)

DOI：10.1016/j.actbio.2024.12.048

Reactivity：Mouse
Bioactive hydrogel synergizes neuroprotection, macrophage polarization, and angiogenesis to improve repair of traumatic brain injury

IF: 8.7

Journal： Materials Today Bio (2024)

DOI：10.1016/j.mtbio.2024.101335

Reactivity：Mouse
Engineered Carrier-Free Nanosystem-Induced In Situ Therapeutic Vaccines for Potent Cancer Immunotherapy

IF: 8.3

Journal： ACS Applied Materials & Interfaces (2024)

DOI：10.1021/acsami.4c09925

Reactivity：Mouse

Sample Type：4T1 cell-Xenograft
PFDA promotes cancer metastasis through macrophage M2 polarization mediated by Wnt/β-catenin signaling

IF: 8.1

Journal： CHEMOSPHERE (2024)

DOI：10.1016/j.chemosphere.2024.142758

Reactivity：Mouse
Enhancing Tumor-Specific immunity with SLdacA: A attenuated Salmonella-mediated c-di-AMP delivery system targeting the STING pathway

IF: 5.3

Journal： INTERNATIONAL JOURNAL OF PHARMACEUTICS (2024)

DOI：10.1016/j.ijpharm.2024.124759

Reactivity：Mouse
Effect of Trichinella spiralis-Derived Antigens on Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in Mice

IF: 4.9

Journal： ACS Pharmacology & Translational Science (2024)

DOI：10.1021/acsptsci.3c00276

Reactivity：Mouse

E-CK-A105	10×ACK Lysis Buffer	500 mL , 200 mL , 100 mL
E-CK-A107	Cell Staining Buffer	500 mL , 200 mL , 100 mL
E-CK-A109	Intracellular Fixation/Permeabilization Buffer Kit	500 Assays , 100 Assays , 50 Assays

Q11:IHC实验，想知道抗原修复条件是什么？是用EDTA还是枸橼酸钾？

修复条件一般为高温高压或者微波加热修复。
例如：抗原修复（高压法）：压力锅中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液（pH 6.0）（配制：将抗原修复液溶于相应体积 ddH2O中，混匀），加热至沸腾，切片置耐热塑料切片架上，放入锅中，盖好锅盖，扣上压力阀，继续加热，设置保压 4 min，时间到后打开放气阀放气，压力归零后开锅盖将内锅取出放置室温冷却，待溶液冷却至室温后取出切片（约30 min）。
抗原修复（微波法）：烧杯中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液（pH 6.0）（配制：将抗原修复液溶于相应体积 ddH2O中，混匀），加热至沸腾，切片置耐热塑料切片架上，放入烧杯中，中小火加热30min后取出，放置室温冷却，待溶液冷却至室温后取出切片（约30 min）。
IHC修复液的选择建议参考抗体使用说明书，或者参考以下方案：人体样本，优先推荐使用pH9.0的EDTA修复液（临床样本）；大/小鼠样本，优先推荐使用pH6.0的枸橼酸修复液（科研样本）。

Q12:IHC在数据表上是什么意思？是冷冻切片吗？与IHC-F区别？

IHC是免疫组化实验，使用的切片一般包含有IHC-P（石蜡切片）和IHC-F（冰冻切片）两种。