Ascorbate Peroxidase (APX) Activity Assay Kit
中文名称：抗坏血酸过氧化物酶（APX）比色法测试盒
Price:
Size:
100 Assays (96 samples)
50 Assays (48 samples)
- 样本类型： 植物组织
- 检测仪器及波长： 紫外-可见光分光光度计（290 nm）
- 研究领域： 植物生理系列
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
抗坏血酸过氧化物酶（APX）催化抗坏血酸（ASA）与过氧化氢（H2O2）反应，使ASA氧化成单脱氢抗坏血酸（MDASA）。随着ASA被氧化，溶液中290nm波长下吸光度值（A290）下降，根据单位时间内A290减少值，计算抗坏血酸过氧化物酶（APX）活性。ASA氧化量按消光系数2.8 mL/（μmol·cm）计算。
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| 指标简称 | APX |
| 灵敏度 | 0.071 U/g tissue |
| 检测范围 | 0.071-47 U/g tissue |
| 精密度 | 批间差：6.4% | 批内差：4.8% |
| 样本量 | 100 μL(tissue homogenate) |
| 检测时间 | 1 h |
| 检测仪器及波长 | 紫外-可见光分光光度计（290 nm） |
| 保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
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