Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
中文名称:谷草转氨酶(AST/GOT)比色法测试盒

Price:
Size:
96 T (43 samples)
48 T (19 samples)
- 样本类型: 血清(浆);动物组织;细胞;细胞上清
- 检测仪器及波长: 酶标仪(500-520 nm)
- 研究领域: 肝肾功能
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
谷草转氨酶(AST)催化天冬氨酸与α-酮戊二酸之间的氨基转换反应,生成谷氨酸和草酰乙酸,草酰乙酸在反应过程中可自行脱羧成丙酮酸。丙酮酸与苯肼反应,生成苯腙,苯腙在碱性条件下呈红棕色。
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指标简称 | AST |
灵敏度 | 1.10 IU/L |
检测范围 | 1.10-72.30 IU/L |
精密度 | 批间差:6.8% | 批内差:5.3% |
样本量 | 10 μL |
检测时间 | 1 h 20 min |
检测仪器及波长 | 酶标仪(500-520 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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E-BC-K235-M | Alanine Aminotransferase (ALT/GPT) Activity Assay Kit | 96 T (42 samples) , 48 T (18 samples) |
Q1:组织样本冻存比较久了,能否检测AST/ALT?
建议尽量使用新鲜的样本,如果没有新鲜样本,建议样本-80℃冻存不超过一个月,否则AST/ALT酶活降低,对测值有较大的影响。

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