B7-2/CD86 Polyclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Rabbit
- 反应性： Human
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB: Romas Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:500-1:1000, IHC 1:200-1:400 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Rat B7-2/CD86 protein expressed by Mammalian |
| Abbre | CD86 |
| Synonyms | Activation B7-2 antigen, B72, B7-2, B-lymphocyte activation antigen B7-2, BU63, CD86 antigen, CD86 molecule, CD86, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86, CD28LG2B7-2 antigen), CD28 antigen ligand 2 |
| Swissprot | |
| Calculated MW | 37KDa |
| Observed MW | 75kDa |
| Cellular Localization | Cell membrane, Secreted |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. |
Western blot with Anti B7-2/CD86 Polyclonal antibody at dilution of 1:1000. Lane 1: Romas cell lysate.
Immunohistochemistry of paraffin-embedded Human tonsil using B7-2/CD86 Polyclonal Antibody at dilution of 1:850.
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