beta actin Monoclonal Antibody
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Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC;IF
| Verified Samples |
Verified Samples in WB: Raji, Jurkat, THP-1, Mouse spleen, Rat spleen, Raw264.7, C6 Verified Samples in IHC: Human stomach cancer, Human lung cancer Verified Samples in IF: U-2 OS, HeLa, NIH/3T3, C6 |
| Dilution | WB 1:2000-1:4000, IHC 1:200-1:400, IF 1:200-1:400 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant human Beta actin protein by E.coli |
| Abbre | beta actin |
| Synonyms | Alpha tubulin 3, Alpha-tubulin 3, B alpha 1, FLJ25113, LIS3, TBA1A, TUBA1A, TUBA3 |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW | 43 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Tags and Cell Markers, Isotype/Loading, Controls, Neuroscience, Biochemicals, Cancer, Cardiovascular, Cell Biology, Immunology, Metabolism, Epigenetics and Nuclear Signaling, Stem Cells |
| Clone No. | 3A9 |
| Conjugation | Unconjugated |
| Background | Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility,structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells,and commonly used as a loading control for western blot and as a control for qPCR. |
Western blot with Anti beta actin Monoclonal Antibody at dilution of 1:3000. Lane 1: Raji cell lysate, Lane 2: Jurkat cell lysate, Lane 3: THP-1 cell lysate, Lane 4: Mouse spleen tissue lysate, Lane 5: Rat spleen tissue lysate, Lane 6: Raw264.7 cell lysate, Lane 7: C6 cell lysate.
Immunohistochemistry of paraffin-embedded Human Stomach cancer using beta actin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human Lung cancer using beta actin Monoclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of U-2 OS cells using beta actin Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of HeLa cells using beta actin Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of NIH/3T3 cells using beta actin Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of C6 cells using beta actin Monoclonal Antibody at dilution of 1:200.
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