beta Tubulin Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat;Zebrafish
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB: HT-29, Jurkat, 293T, Raw264.7, C6, Rat brain, Zebrafish Verified Samples in IF: U-2 OS |
| Dilution | WB 1:2000-1:4000, IF 1:200-1:400 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Recombinant human TUBB protein by E.coli |
| Abbre | beta Tubulin |
| Synonyms | UBB 1, TUBB 2, TUBB 5, TUBB, TUBB1, TUBB2 |
| Swissprot | |
| Calculated MW | 55 kDa |
| Observed MW | 55 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton |
| Tissue Specificity | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Neuroscience, Tags and Cell Markers, Isotype/Loading Controls, Biochemicals, Cancer, Cardiovascular, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Immunology, Metabolism, Microbiology, Stem Cells |
| Clone No. | 2E8-2 |
| Conjugation | Unconjugated |
| Background | In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules,a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes,including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot. |
Western blot with Anti beta Tubulin Monoclonal Antibody at dilution of 1:3000. Lane 1: HT-29 cell lysate, Lane 2: Jurkat cell lysate, Lane 3: 293T cell lysate, Lane 4: Raw264.7 cell lysate, Lane 5: C6 cell lysate, Lane 6: Rat brain tissue lysate, Lane 7: Zebrafish tissue lysate.
Immunofluorescence analysis of U-2 OS cells using beta Tubulin Monoclonal Antibody at dilution of 1:200.
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The Role of LC3-Associated Phagocytosis Inhibits the Inflammatory Response in Aspergillus fumigatus Keratitis
IF: 5.000Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2024)Reactivity：MouseSample Type：corneas tissue -
Resveratrol has neuroprotective effects and plays an anti-inflammatory role through Dectin-1/p38 pathway in Aspergillus fumigatus keratitis
IF: 3.700 -
Chelerythrine ameliorates Aspergillus fumigatus keratitis through suppressing the LOX-1/p38 MAPK signaling pathway
IF: 3.700 -
Increased expression of CB2 receptor in the intestinal biopsies of children with inflammatory bowel disease
IF: 3.953Journal： PEDIATRIC RESEARCH (2022)Reactivity：HumanSample Type：ileum ,rectum tissue -
Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation
IF: 3.470Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2020)Reactivity：Human
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