Blocking Powder
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- 应用性： WB;IHC
| 产品简介 | 本产品纯度高，为淡黄白色粉末。
本产品应用范围广，常用于免疫相关实验，如用于Western、ELISA和免疫染色的封闭、一抗或二抗的稀释等。此外，本品也可以作为蛋白浓度定量检测的标准品。 本产品在PBS中的溶解度大于50 mg/mL，确保可以配制成透明澄清的5% BSA溶液。 |
| 保存条件 | 2~8°C保存，有效期12个月。 |
| 注意事项 | 1.本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 2.为了您的安全和健康，请穿实验服并戴一次性手套操作。 |
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| E-IR-R107 | 5% Blocking Buffer | 50 mL , 10 mL , 5 mL |
Q1:请问BSA室温下封闭多久？
封闭操作时间通常为1.5小时。
Q2:E-IR-R108 BSA的化学规格？
牛血清中的简单蛋白，是血液的主要成分（38g/1000ml），分子量68kD。等电点4.8。牛血清白蛋白在生化实验中有广泛的应用，例如在western blot中作为Blocking buffer。客户那边如果是用来做为ELISA实验的封闭蛋白，能否适用具体要看客户的实验体系，不同的BSA对于ELISA实验封闭来说区别很大，客户可以通过预实验来判断。
Q3:BSA做WB实验推荐什么浓度呢？
用TBST稀释，BSA浓度 2-5%。
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