Ca2+-ATPase Activity Assay Kit
中文名称:Ca2+-ATP酶比色法测试盒

Price:
Size:
96 T (46 samples)
48 T (22 samples)
- 样本类型: 动物组织
- 检测仪器及波长: 酶标仪(660 nm)
- 研究领域: 氧化应激
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
ATP酶可分解ATP生成ADP及无机磷,测定无机磷的量可判断ATP酶活力的高低。在对照体系中通过抑制Ca2+-ATP酶酶活,测定体系不抑制Ca2+-ATP酶酶活,测定与对照两者无机磷含量差值即为Ca2+-ATP酶在孵育时间内产生的无机磷。通过无机磷产生判断Ca2+-ATP酶的酶活。
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灵敏度 | 1.18 U/kg wet weight |
检测范围 | 1.18-286.43 U/kg wet weight |
精密度 | 批间差:0.08 | 批内差:0.06 |
样本量 | 400 μL(tissue homogenate) |
检测时间 | 1 h 30 min |
检测仪器及波长 | 酶标仪(660 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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