Calcium (Ca) Colorimetric Assay Kit
中文名称:钙(Ca)比色法测试盒

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);尿液;组织
- 检测仪器及波长: 酶标仪(600-620 nm,最佳检测波长为610 nm)
- 研究领域: 无机盐离子;植物生理系列
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
样本中钙离子在碱性溶液中与甲基百里香酚蓝(MTB)结合,生成蓝色络合物;其颜色深浅与钙离子浓度成正比,该物质在610 nm处有最大吸收峰,通过比色与同样处理的钙标准进行比较,可计算出样本中钙的含量。
|
指标简称 | Ca |
灵敏度 | 0.07 mmol/L |
检测范围 | 0.07-1.2 mmol/L |
精密度 | 批间差:8.5% | 批内差:4.7% |
样本量 | 10 μL |
检测时间 | 20 min |
检测仪器及波长 | 酶标仪(600-620 nm,最佳检测波长为610 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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