Cell Ferrous Iron Colorimetric Assay Kit
中文名称:细胞亚铁比色法测试盒

Price:
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(590-600 nm,最佳检测波长为593 nm)
- 研究领域: 无机盐离子;铁死亡
检测原理 |
铁是生物体内重要的金属元素之一,具有重要的生理作用。亚铁离子是血红素和血红蛋白中的关键元素,同时也在许多生化反应中起到重要作用。样本中的亚铁离子与探针结合,生成的物质在593 nm波长处有强吸收峰,在一定范围内其光密度值与亚铁离子浓度成线性相关。
|
指标简称 | Fe2+ |
灵敏度 | 0.4 μmol/L |
检测范围 | 0.4-50 μmol/L |
精密度 | 批间差:1.5% | 批内差:1.3% |
样本量 | 160 μL(cell homogenate) |
检测时间 | 40 min |
检测仪器及波长 | 酶标仪(590-600 nm,最佳检测波长为593 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
-
Circular RNA circBNC2 inhibits tumorigenesis by modulating ferroptosis and acts as a nanotherapeutic target in prostate cancer
IF: 27.700 -
Antioxidant taurine inhibits chondrocyte ferroptosis through upregulation of OGT/Gpx4 signaling in osteoarthritis induced by anterior cruciate ligament transection
IF: 11.400 -
Tungsten-based polyoxometalate nanoclusters as ferroptosis inhibitors modulating S100A8/A9-mediated iron metabolism pathway for managing intracerebral haemorrhage
IF: 10.600 -
EBF1-induced CSRP2 boosts the progression of B-cell acute lymphocytic leukemia by inhibiting ferroptosis
IF: 9.100 -
Targeted inhibition of ferroptosis in bone marrow mesenchymal stem cells by engineered exosomes alleviates bone loss in smoking-related osteoporosis
IF: 8.700 -
METTL3-mediated m6A modification of SLC7A11 enhances nasopharyngeal carcinoma radioresistance by inhibiting ferroptosis
IF: 8.200Journal: International Journal of Biological Sciences (2025)Reactivity:Human -
TREM2 alleviates sepsis-induced acute lung injury by attenuating ferroptosis via the SHP1/STAT3 pathway
IF: 7.100Journal: FREE RADICAL BIOLOGY AND MEDICINE (2025)Reactivity:MouseSample Type:lung tissue -
Maternal exposure to deltamethrin during pregnancy and lactation impairs hippocampal learning and memory function of male offspring by ferroptosis
IF: 6.200Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2025)Reactivity:Rat,MouseSample Type:hippocampus tissue -
Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunction
IF: 6.200Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2025)Reactivity:Chicken -
IDO1 inhibits ferroptosis by regulating FTO-mediated m6A methylation and SLC7A11 mRNA stability during glioblastoma progression
IF: 6.100
E-BC-K028-M | Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples) | 96 T (92 samples) , 48 T (44 samples) |
E-BC-K030-M | Reduced Glutathione (GSH) Colorimetric Assay Kit | 96 T (79 samples) , 48 T (31 samples) |
E-BC-K138-F | Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green) | 96 T |
E-BC-K880-M | Cell Total Iron Colorimetric Assay Kit | 96 T (80 samples) , 48 T (32 samples) |
Q1:细胞样本如何进行破碎处理?
本试剂盒使用配套细胞裂解液对细胞样本进行处理,加入裂解液后,只需在冰盒上静置10分钟,即可离心取上清液待测,有效提升了细胞破碎效率。
Q2:最后的结果计算可以不用细胞数量,而是蛋白浓度进行计算吗?
样本处理过程中所用到的试剂一会对蛋白浓度测定有影响,因此不能用蛋白浓度计算,而是用细胞数进行计算。
Q3:E-BC-K881-M这个试剂盒里面操作说明提到的对照孔的作用是什么?每个样本都需要一个对照孔吗?
这个试剂盒对照孔设置的目的是排除样本本身的干扰。每个样本都至少需要一个对照孔和一个测定孔。