Cell Total Iron Colorimetric Assay Kit
中文名称:细胞总铁比色法测试盒

Price:
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(590-600 nm,最佳检测波长为593 nm)
- 研究领域: 无机盐离子;铁死亡
检测原理 |
铁是生物体内重要的金属元素之一,具有重要的生理作用。亚铁离子是血红素和血红蛋白中的关键元素,同时也在许多生化反应中起到重要作用。在还原剂的作用下,样本中的铁离子被还原为亚铁离子,亚铁离子与探针结合,生成的物质在593 nm波长处有强吸收峰,在一定范围内其光密度值与铁离子浓度成线性相关。
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灵敏度 | 0.4 μmol/L |
检测范围 | 0.4-50 μmol/L |
精密度 | 批间差:1.5% | 批内差:1.3% |
样本量 | 80 μL(cell homogenate) |
检测时间 | 1 h 20 min |
检测仪器及波长 | 酶标仪(590-600 nm,最佳检测波长为593 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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Q1:细胞样本如何进行破碎处理?
本试剂盒使用配套细胞裂解液对细胞样本进行处理,加入裂解液后,只需在冰盒上静置10分钟,即可离心取上清液待测,有效提升了细胞破碎效率。
Q2:最后的结果计算可以不用细胞数量,而是蛋白浓度进行计算吗?
样本处理过程中所用到的试剂一会对蛋白浓度测定有影响,因此不能用蛋白浓度计算,而是用细胞数进行计算。
Q3:总铁试剂盒中的试剂一可以裂解细菌吗?革兰阳性菌。
这个试剂盒中的裂解液不能裂解细菌,因为该试剂的裂解强度不够。