Cell Ferrous Iron (Fe2+ ) Fluorometric Assay Kit
中文名称:细胞亚铁离子(Fe2+ )荧光法测试盒

Price:
Size:
96 T
- 样本类型: 细胞
- 检测仪器及波长: 荧光酶标仪(Ex/Em=542 nm/575 nm)、流式细胞仪(Ex/Em=542 nm/575 nm)、荧光显微镜(Ex/Em=542 nm/570-620 nm)
- 研究领域: 无机盐离子;铁死亡
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
研究证实铁是生物体内量最多的过渡金属元素,其参与多种生理活动。近几年,细胞内的游离铁离子由于具有很高的反应性,和细胞损伤、死亡有一定的关联而得到了越来越多的关注。在细胞内游离铁离子以稳定的Fe2+和 Fe3+形式存在。从细胞内的还原环境、金属转运体及Fe2+的水溶性考虑,因此揭示细胞内Fe2+的行为比Fe3+更重要。
本试剂盒提供一种荧光探针可以和Fe2+特异性结合,探针可以很好的进入到细胞内部,适用于活细胞内Fe2+的检测。当探针与Fe2+反应后,可以生成一种不可逆的橙色(红色)荧光产物(激发波长:542 nm,发射波长:575 nm)。 |
指标简称 | Fe2+ |
检测时间 | 1 h 30 min |
检测仪器及波长 | 荧光酶标仪(Ex/Em=542 nm/575 nm)、流式细胞仪(Ex/Em=542 nm/575 nm)、荧光显微镜(Ex/Em=542 nm/570-620 nm) |
保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 6个月 |
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Q1:如果实验处理中用到的药物也是一种铁螫合剂,试剂盒原理也是铁螫合剂,这样会不会存在一种竞争关系,导致检测的结果变化?
如果您的实验目的是验证这个药物(螯合剂)的作用,那就是可以用的。这个试剂盒的探针就是检测没有被螯合的铁离子,探针不会去抢已经螯合的铁。
Q2:亚铁荧光法试剂盒,该探针是否能够跟DAPI共染?
有文献记录该探针可以和DAPI共染