CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
中文名称:分型超氧化物歧化酶(CuZn-SOD/Mn-SOD)测试盒(羟胺法)

Price:
Size:
100 Assays (50 samples)
50 Assays (25 samples)
- 样本类型: 血清(浆);尿液;细胞;细胞上清;动植物组织
- 检测仪器及波长: 紫外-可见光分光光度计(550 nm)
- 研究领域: 氧化应激
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基(O2-),它氧化羟胺形成亚硝酸盐,在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后,催化超氧阴离子自由基发生歧化反应,使形成的亚硝酸盐减少,进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值,来计算SOD的活性。高等生物细胞内含有有铜锌-SOD(CuZn-SOD)和锰-SOD(Mn-SOD)同时,经样本前处理过程的样本中Mn-SOD活力丧失,但CuZn-SOD活力不变。
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指标简称 | CuZn-SOD |
灵敏度 | 2.03 U/mL |
检测范围 | 2.03-155 U/mL |
精密度 | 批间差:7.2% | 批内差:4.0% |
样本量 | 20-50 μL |
检测时间 | 1 h 40 min |
检测仪器及波长 | 紫外-可见光分光光度计(550 nm) |
保存条件 | 该产品可在 2-8°C/-20°C避光条件下保存6个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 6个月 |
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E-BC-K019-S | Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method) | 100 Assays (50 samples) , 50 Assays (25 samples) |

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