CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
中文名称：分型超氧化物歧化酶（CuZn-SOD/Mn-SOD）测试盒（羟胺法）
Price:
Size:
100 Assays (50 samples)
50 Assays (25 samples)
- 样本类型： 血清（浆）;尿液;细胞;细胞上清;动植物组织
- 检测仪器及波长： 紫外-可见光分光光度计（550 nm）
- 研究领域： 氧化应激
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基（O2-），它氧化羟胺形成亚硝酸盐，在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后，催化超氧阴离子自由基发生歧化反应，使形成的亚硝酸盐减少，进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值，来计算SOD的活性。高等生物细胞内含有有铜锌-SOD（CuZn-SOD）和锰-SOD（Mn-SOD）同时，经样本前处理过程的样本中Mn-SOD活力丧失，但CuZn-SOD活力不变。
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| 指标简称 | CuZn-SOD |
| 灵敏度 | 2.03 U/mL |
| 检测范围 | 2.03-155 U/mL |
| 精密度 | 批间差：7.2% | 批内差：4.0% |
| 样本量 | 20-50 μL |
| 检测时间 | 1 h 40 min |
| 检测仪器及波长 | 紫外-可见光分光光度计（550 nm） |
| 保存条件 | 该产品可在 2-8°C/-20°C避光条件下保存6个月，具体试剂组分的保存条件请参考说明书。 |
| 有效期 | 6个月 |
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| E-BC-K019-S | Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method) | 100 Assays (50 samples) , 50 Assays (25 samples) |
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