D-Lactic Acid/Lactate(LA)Colorimetric Assay Kit
中文名称：D-乳酸（LA）比色法测试盒
Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型： 血清（浆）;唾液;动物组织
- 检测仪器及波长： 酶标仪（520-550 nm，最佳检测波长为530 nm）
- 研究领域： 糖脂代谢
为了简化计算过程，可下载该产品计算文件。如使用中有任何问题，请随时联系技术支持
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
以氧化型辅酶Ⅰ（NAD+）为氢受体，D-乳酸脱氢酶（LDH）催化D-乳酸脱氢产生丙酮酸，使NAD+转化为还原型辅酶Ⅰ（NADH）。其中N-甲基吩嗪甲基硫酸盐（PMS）递氢使氯化硝基四氮唑蓝（NBT）还原为紫色呈色物，呈色物的吸光度在530 nm时与乳酸含量呈线性关系。整个过程中，L-乳酸不影响测定结果，因此，通过测定波长530 nm处吸光度值的变化，即可计算出样本中D-乳酸的含量。
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| 指标简称 | D-LA |
| 灵敏度 | 0.06 mmol/L |
| 检测范围 | 0.06-8.0 mmol/L |
| 精密度 | 批间差：7.7% | 批内差：3.8% |
| 样本量 | 5 μL |
| 检测时间 | 20 min |
| 检测仪器及波长 | 酶标仪（520-550 nm，最佳检测波长为530 nm） |
| 保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
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| E-BC-K044-M | L-Lactic Acid (LA) Colorimetric Assay Kit | 96 T (80 samples) , 48 T (32 samples) |
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