D-Lactic Acid/Lactate(LA)Colorimetric Assay Kit
中文名称:D-乳酸(LA)比色法测试盒

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);唾液;动物组织
- 检测仪器及波长: 酶标仪(520-550 nm,最佳检测波长为530 nm)
- 研究领域: 糖脂代谢
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
以氧化型辅酶Ⅰ(NAD+)为氢受体,D-乳酸脱氢酶(LDH)催化D-乳酸脱氢产生丙酮酸,使NAD+转化为还原型辅酶Ⅰ(NADH)。其中N-甲基吩嗪甲基硫酸盐(PMS)递氢使氯化硝基四氮唑蓝(NBT)还原为紫色呈色物,呈色物的吸光度在530 nm时与乳酸含量呈线性关系。整个过程中,L-乳酸不影响测定结果,因此,通过测定波长530 nm处吸光度值的变化,即可计算出样本中D-乳酸的含量。
|
指标简称 | D-LA |
灵敏度 | 0.06 mmol/L |
检测范围 | 0.06-8.0 mmol/L |
精密度 | 批间差:7.7% | 批内差:3.8% |
样本量 | 5 μL |
检测时间 | 20 min |
检测仪器及波长 | 酶标仪(520-550 nm,最佳检测波长为530 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
-
A preventative role of nitrate for hypoxia-induced intestinal injury
IF: 7.1Journal: FREE RADICAL BIOLOGY AND MEDICINE (2024)Reactivity:MouseSample Type:serum -
Exopolysaccharides and Surface-Layer Proteins Expressed by Biofilm-State Lactiplantibacillus plantarum Y42 Play Crucial Role in Preventing Intestinal Barrier and Immunity Dysfunction of Balb/C Mice Infected by Listeria monocytogenes ATCC 19115
IF: 5.7Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity:MouseSample Type:serum -
Fucoxanthin alleviates lipopolysaccharide-induced intestinal barrier injury in mice
IF: 5.1 -
Bacillus halotolerans attenuates inflammation induced by enterotoxigenic Escherichia coli infection in vivo and in vitro based on its metabolite soyasaponin I regulating the p105-Tpl2-ERK pathway
IF: 5.1 -
Paper-Based Microfluidic Device for Extracellular Lactate Detection
IF: 4.9 -
Pterostilbene Induces Pyroptosis in Breast Cancer Cells through Pyruvate Kinase 2/Caspase-8/Gasdermin C Signaling Pathway
IF: 4.9Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity:MouseSample Type:EMT6 cell-Xenograft,4T1 cell-Xenograft -
Partially hydrolyzed guar gum attenuates broad-spectrum antibiotic-induced gut microbiota disruption associated with intestinal inflammation and barrier damage in mice
IF: 4.8 -
Protective effect of a cocktail of lactic acid bacteria on DSS-induced ulcerative colitis via activating the TGR5/PKA pathway
IF: 4.8 -
Detection of serum lactate dehydrogenase A and its metabolites on placental function in patients with intrahepatic cholestasis of pregnancy
IF: 4.8Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity:HumanSample Type:serum -
Rehydration effect of qingshu buye decoction on exercise and high temperature-induced dehydration
IF: 4.5Journal: Journal of the International Society of Sports Nutrition (2024)Reactivity:HumanSample Type:serum
E-BC-K044-M | L-Lactic Acid (LA) Colorimetric Assay Kit | 96 T (80 samples) , 48 T (32 samples) |

暂无文件,请在搜索框输入识别码查询相关文件

名称 | 状态 | 有效期至 | 操作 | |
---|---|---|---|---|
{{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注:请在有效期内下载相关文件,过期后页面内将不提供文件下载及预览!
在线咨询