DAB Detection Kit
中文名称：DAB显色试剂盒
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- 应用性： IHC
| 产品简介 | DAB即3,3N-Diaminobenzidine Tertrahydrochloride，是辣根过氧化物酶(HRP)的常用底物，在辣根过氧化物酶的催化下，DAB会产生棕色沉淀。 |
| 保存条件 | 2~8°C避光保存，不得冻存，有效期12 个月。 使用时即拿即放，单支试剂使用后应立即放回冰箱，开封后试剂有效期 6 个月。 |
| 注意事项 | 1. 本显色液主要用于免疫组化显色，不推荐用于其他实验用途。 2. 本染色液仅限专业人员使用。 3. DAB 为联苯偶氮类染料，为可疑致癌物，使用过程中请穿实验服并戴一次性手套防护。 4. 滴加适量新鲜配制的 DAB 显色工作液，室温孵育 5~10 分钟。控制好 DAB 显色时间，阳性信号为 棕黄色或褐黄色，切勿显色过深。 5. 蒸馏水冲洗切片终止显色后复染、脱水、透明、封片。 6. 为防止可能出现的假阳性、假阴性结果，在实验过程中请设置阳性与阴性对照。 7. 实验中滴加试剂时，PBS 缓冲液会导致试剂被稀释，将引起染色强度变弱，因此，滴加试剂前请除 去多余的缓冲液。 |
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