DAPI Reagent (1 μg/mL)
中文名称:DAPI染色液

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- 应用性: IF
产品简介 | DAPI即4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole),是一种能够与DNA强力结合的荧光染料,常用荧光显微镜观测。DAPI可以透过完整的细胞膜,可用于活细胞和固定细胞的染色。CAS Number 28718-90-3。
DAPI可以穿透细胞膜与细胞核中的双链DNA结合,可以产生比DAPI自身强20多倍的荧光,和EB相比,DAPI对双链DNA的染色灵敏度要高很多倍。显微镜下可以看到呈蓝色荧光的细胞,荧光显微镜观察细胞标记的效率高(几乎为100%) ,且DAPI对活细胞无毒副作用。 DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA着色。细胞经热激处理后用DAPI染色3分钟,在荧光显微镜下可以看到细胞核的形态发生变化。 DAPI染色液的最大激发波长为340 nm,最大发射波长为488 nm,DAPI和双链DNA结合后,最大激发波长为360 nm,最大发射波长为460 nm。 本DAPI染色液可以直接用于固定细胞或组织的细胞核染色。 |
保存条件 | 2~8°C避光保存,有效期12个月。 |
注意事项 | 1.荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。 2.为减缓荧光淬灭可以使用抗荧光淬灭封片液。 3.使用过程中请穿实验服并戴一次性手套防护。 |
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