Elab Fluor® 647-conjugated alpha Tubulin Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: Human
- 应用性: FCM
Verified Samples |
Verified Samples in FCM: HepG2 cells were transfected with an expression plasmid to prepare an overexpression cell model |
Dilution | FCM 0.2 μg per 106 cells in a 100 μL suspension |
Clonality | Monoclonal |
Isotype | IgG2a |
Immunogen | Recombinant human TUBA1B protein by E. Coli |
Abbre | alpha Tubblin |
Synonyms | Alpha tubulin 3, Alpha-tubulin 3, B alpha 1, FLJ25113, LIS3, TBA1A, TUBA1A, TUBA3, Tubulin B alpha 1, Tubulin B-, Tubulin alpha 1A chain, Tubulin alpha 1a, Tubulin alpha 3, Tubulin alpha 3 chain, Tubulin alpha brain specific, Tubulin alpha-1A chain, Tubulin alpha-3 chain |
Swissprot | |
Cellular Localization | Cytoplasm, cytoskeleton. |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Research Areas | Signal Transduction, Neuroscience, Tags and Cell Markers, Isotype/Loading Controls, Biochemicals, Cancer, Cardiovascular, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Immunology, Metabolism, Microbiology, Stem Cells |
Clone No. | 2H2 |
Conjugation | Elab Fluor®647 |
Amax | 650nm |
Emax | 665nm |
Background | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers .Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms.Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Elab Fluor® 647-conjugated alpha Tubulin Monoclonal Antibody (red), or 0.2 ug Elab Fluor® 647-conjugated alpha Tubulin Monoclonal Antibody Isotype Control (green).