FITC-conjugated beta Tubulin Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: Human
- 应用性: FCM
Verified Samples |
Verified Samples in FCM: Hela cells were transfected with an expression plasmid to prepare an overexpression cell model |
Dilution | FCM 0.2 μg per 106 cells in a 100 μL suspension |
Clonality | Monoclonal |
Isotype | IgG |
Immunogen | Recombinant human TUBB protein by E. Coli |
Abbre | beta Tubulin |
Synonyms | Beta 4 tubulin, Beta 5 tubulin, Beta1 tubulin, Class I beta tubulin, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, Tu, Tubulin beta 2 chain, Tubulin beta chain, Tubulin beta class I, beta Ib tubulin, tubulin beta 1 chain, tubulin beta 5 chain, tubulin beta polypeptide |
Swissprot | |
Cellular Localization | Cytoplasm, Cytoskeleton, Microtubule. |
Concentration | 1 mg/mL |
Storage | Store at 4°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
Buffer | PBS with 0.05% proclin 300 and 0.1% gelatin,pH7.4 |
Purification Method | Protein A/G Purification |
Research Areas | Signal Transduction, Neuroscience, Tags and Cell Markers, Isotype/Loading Controls, Biochemicals, Cancer, Cardiovascular, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Immunology, Metabolism, Microbiology, Stem Cells |
Clone No. | 2E8-2 |
Conjugation | FITC |
Amax | 495nm |
Emax | 525nm |
Background | In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules,a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes,including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot. |
1X10^6 Hela cells were intracellularly stained with 0.2 ug FITC-conjugated beta Tubulin Monoclonal Antibody (red), or 0.2 ug FITC-conjugated beta Tubulin Monoclonal Antibody Isotype Control (green).