FITC-conjugated HA-Tag Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: All
- 应用性: FCM
Verified Samples |
Verified Samples in FCM: 293F cells were transfected with a tag expression plasmid to prepare an overexpression cell model |
Dilution | FCM 0.2 μg per 106 cells in a 100 μL suspension |
Clonality | Recombinant |
Isotype | IgG2a |
Immunogen | HA tag fusion protein |
Abbre | HA Tag |
Synonyms | HA epitope tag, HA1, HA2, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, hemagglutinin |
Concentration | 1 mg/mL |
Storage | Store at 4°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
Buffer | PBS with 0.05% proclin 300 and 0.1% gelatin,pH7.4 |
Purification Method | Protein A/G Purification |
Research Areas | Signal Transduction, Immunology , Cancer, Metabolism, Cardiovascular, Epigenetics and Nuclear Signaling, Cell Biology, Tags and Cell Markers, Microbiology, Stem Cells Neuroscience, Secondary antibodies, Isotype/Loading Controls, Biochemicals, Developmental Biology |
Clone No. | 4F5 |
Conjugation | FITC |
Amax | 495nm |
Emax | 525nm |
Background | The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag,which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
1X10^6 HEK-293F cells were intracellularly stained with 0.2 ug FITC-conjugated HA-Tag Monoclonal Antibody (red), or 0.2 ug FITC-conjugated HA-Tag Monoclonal Antibody Isotype Control (green).