Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit (Enzyme Method)
中文名称:游离脂肪酸(NEFA/FFA) 比色法测试盒(酶法)

Price:
Size:
96 T (92 samples)
48 T (44 samples)
- 样本类型: 血清(浆);动物组织;细胞
- 检测仪器及波长: 酶标仪(540-550 nm,最佳检测波长为546 nm)
- 研究领域: 糖脂代谢;代谢疾病;能量代谢
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
游离脂肪酸(NEFA/FFA)在酰基合成酶的作用下生成酰基辅酶 A, 酰基辅酶 A 在酰基氧化酶的氧化作用下生成过氧化氢。过氧化氢在酶和色原的存在下反应,生成红色物质,在 546 nm 处有最大吸收,颜色深浅与与游离脂肪酸浓度成正比。
|
指标简称 | NEFA |
灵敏度 | 0.14 mmol/L |
检测范围 | 0.14-4 mmol/L |
精密度 | 批间差:2.5-6.1% | 批内差:1.5-4.5% |
样本量 | 10 μL |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(540-550 nm,最佳检测波长为546 nm) |
保存条件 | 该产品可在 2-8°C/-20°C避光条件下保存6个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 6个月 |
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