Glutamic Acid Colorimetric Assay Kit
中文名称:谷氨酸比色法测试盒

Price:
Size:
100 Assays (96 samples)
50 Assays (46 samples)
- 样本类型: 血清(浆);动物组织;细胞;细胞上清
- 检测仪器及波长: 紫外-可见光分光光度计(340 nm)
- 研究领域: 氨基酸与蛋白;铁死亡
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
谷氨酸和NAD+在谷氨酸脱氢酶作用下产生α-酮戊二酸、NADH和NH4+,NADH在340 nm有最大吸收,通过测定NADH的变化,可计算谷氨酸的含量。
|
灵敏度 | 4.00 μmol/L |
检测范围 | 4.00-450 μmol/L |
精密度 | 批间差:2.9% | 批内差:2.2% |
样本量 | 0.2 mL |
检测时间 | 1 h |
检测仪器及波长 | 紫外-可见光分光光度计(340 nm) |
保存条件 | 该产品可在 2-8°C/-20°C避光条件下保存3个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 3个月 |
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