Glutathione-S-Transferase (GST) Activity Assay Kit(DTNB method)
中文名称:谷胱甘肽S-转移酶(GST)比色法测试盒(DTNB法)

Price:
Size:
96 T (40 samples)
- 样本类型: 血清(浆);动物组织
- 检测仪器及波长: 酶标仪(400-420 nm,最佳检测波长为412 nm)
- 研究领域: 氧化应激;肝肾功能
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
谷胱甘肽S-转移酶(GST) 具有催化还原型谷胱甘肽(GSH) 与二硝基苯(CDNB) 结合的能力,通过测定底物GSH在单位时间内与二硝基苯结合的速率来表示酶的活力,反应剩余的GSH与二硫代双二硝基苯甲酸(DTNB) 作用生成的黄色硫代硝基苯甲酸阴离子(TNB) ,测定该阴离子浓度来计算出GSH的减少量。从而推算出谷胱甘肽S-转移酶(GST) 的酶活力。
|
指标简称 | GST |
灵敏度 | 2.1 U/L |
检测范围 | 2.1-92.8 U/L |
精密度 | 批间差:6.4% | 批内差:1.8% |
样本量 | 40 μL |
检测时间 | 1 h 10 min |
检测仪器及波长 | 酶标仪(400-420 nm,最佳检测波长为412 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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