Glutathione Peroxidase (GSH-Px) Activity Assay Kit

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中文名称：谷胱甘肽过氧化物酶（GSH-Px）比色法测试盒

货号：E-BC-K096-M
文献（189）
说明书

说明书

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Price: ¥860.00 ¥480.00

Size:

96 T (40 samples) 48 T (16 samples)

样本类型： 血清（浆）;动植物组织;细胞;培养液
检测仪器及波长： 酶标仪（400-420 nm，最佳检测波长为412 nm）
研究领域： 氧化应激;植物生理系列;铁死亡

为了简化计算过程，可下载该产品计算文件。如使用中有任何问题，请随时联系技术支持

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	GSH-Px可以促进过氧化氢（H₂O₂）与还原型谷胱甘肽（GSH）反应生成H₂O及氧化型谷胱甘肽（GSSG），反应式见下图。通过测定底物GSH在单位时间内被氧化的速度来表示酶的活力，反应剩余的GSH与二硫代双二硝基苯甲酸（DTNB）作用生成的黄色硫代硝基苯甲酸阴离子（TNB），测定该阴离子的浓度来计算出GSH的减少量。由于GSH在没有酶的条件下也能进行氧化（非酶反应），所以最后计算此酶活力时，必须扣除非酶反应引起的GSH减少的那部分。
指标简称	GSH-Px
灵敏度	34.34 U
检测范围	34.34-1036.64 U
精密度	批间差：8.7% \| 批内差：2.4%
样本量	40 μL
检测时间	40 min
检测仪器及波长	酶标仪（400-420 nm，最佳检测波长为412 nm）
保存条件	该产品可在 2-8°C避光条件下保存6个月。
有效期	6个月

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