Glutathione Peroxidase (GSH-Px) Activity Assay Kit
中文名称：谷胱甘肽过氧化物酶（GSH-Px）比色法测试盒
Price:
Size:
96 T (40 samples)
48 T (16 samples)
- 样本类型： 血清（浆）;动植物组织;细胞;培养液
- 检测仪器及波长： 酶标仪（400-420 nm，最佳检测波长为412 nm）
- 研究领域： 氧化应激;植物生理系列;铁死亡
为了简化计算过程，可下载该产品计算文件。如使用中有任何问题，请随时联系技术支持
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
生化试剂盒数量计算器x
需测样本数：
单个样本测量孔数：
试剂盒测样本孔数：
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量：
| 检测原理 |
GSH-Px可以促进过氧化氢（H2O2）与还原型谷胱甘肽（GSH）反应生成H2O及氧化型谷胱甘肽（GSSG），反应式见下图。通过测定底物GSH在单位时间内被氧化的速度来表示酶的活力，反应剩余的GSH与二硫代双二硝基苯甲酸（DTNB）作用生成的黄色硫代硝基苯甲酸阴离子（TNB），测定该阴离子的浓度来计算出GSH的减少量。由于GSH在没有酶的条件下也能进行氧化（非酶反应），所以最后计算此酶活力时，必须扣除非酶反应引起的GSH减少的那部分。
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| 指标简称 | GSH-Px |
| 灵敏度 | 34.34 U |
| 检测范围 | 34.34-1036.64 U |
| 精密度 | 批间差：8.7% | 批内差：2.4% |
| 检测时间 | 40 min |
| 检测仪器及波长 | 酶标仪（400-420 nm，最佳检测波长为412 nm） |
| 保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
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