Glutathione Peroxidase (GSH-Px) Activity Assay Kit

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中文名称：谷胱甘肽过氧化物酶（GSH-Px）比色法测试盒

货号：E-BC-K096-S
文献（163）
说明书

说明书

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Price: ¥680.00 ¥380.00

Size:

100 Assays (48 samples) 50 Assays (24 samples)

样本类型： 血清（浆）;动植物组织;细胞;培养液
检测仪器及波长： 紫外-可见光分光光度计（412 nm）
研究领域： 氧化应激;植物生理系列;铁死亡

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	GSH-Px可以促进过氧化氢（H₂O₂）与还原型谷胱甘肽（GSH）反应生成H₂O及氧化型谷胱甘肽（GSSG），反应式如下图。通过测定底物GSH在单位时间内被氧化的速度来表示酶的活力，反应剩余的GSH与二硫代双二硝基苯甲酸（DTNB）作用生成的黄色硫代硝基苯甲酸阴离子（TNB），测定该阴离子的浓度来计算出GSH的减少量。由于GSH在没有酶的条件下也能进行氧化（非酶反应），所以最后计算此酶活力时，必须扣除非酶反应引起的GSH减少的那部分。
指标简称	GSH-Px
灵敏度	12.65 U
检测范围	12.65-387 U
精密度	批间差：9.3% \| 批内差：4.9%
样本量	0.1-0.2 mL
检测时间	1 h 10 min
检测仪器及波长	紫外-可见光分光光度计（412 nm）
保存条件	该产品可在 2-8°C避光条件下保存6个月。
有效期	6个月

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