Glutathione Peroxidase (GSH-Px) Activity Assay Kit
中文名称：谷胱甘肽过氧化物酶（GSH-Px）比色法测试盒
Price:
Size:
100 Assays (48 samples)
50 Assays (24 samples)
- 样本类型： 血清（浆）;动植物组织;细胞;培养液
- 检测仪器及波长： 紫外-可见光分光光度计（412 nm）
- 研究领域： 氧化应激;植物生理系列;铁死亡
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
GSH-Px可以促进过氧化氢（H2O2）与还原型谷胱甘肽（GSH）反应生成H2O及氧化型谷胱甘肽（GSSG），反应式如下图。通过测定底物GSH在单位时间内被氧化的速度来表示酶的活力，反应剩余的GSH与二硫代双二硝基苯甲酸（DTNB）作用生成的黄色硫代硝基苯甲酸阴离子（TNB），测定该阴离子的浓度来计算出GSH的减少量。由于GSH在没有酶的条件下也能进行氧化（非酶反应），所以最后计算此酶活力时，必须扣除非酶反应引起的GSH减少的那部分。
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| 指标简称 | GSH-Px |
| 灵敏度 | 12.65 U |
| 检测范围 | 12.65-387 U |
| 精密度 | 批间差：9.3% | 批内差：4.9% |
| 样本量 | 0.1-0.2 mL |
| 检测时间 | 1 h 10 min |
| 检测仪器及波长 | 紫外-可见光分光光度计（412 nm） |
| 保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
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